Author: Qing, Enya; Hantak, Michael; Perlman, Stanley; Gallagher, Tom
Title: Distinct Roles for Sialoside and Protein Receptors in Coronavirus Infection Document date: 2020_2_11
ID: 1mowsbjy_28
Snippet: Hemagglutination assay. Serial twofold dilutions of 100ϫ concentrated virus and pseudovirus particle stocks were prepared using FBS-free DMEM as diluent. Diluted samples were placed into V-bottom 96-well plates and incubated for 2 h at 4°C. Washed adult human erythrocytes (0.5%) (in phosphate-buffered saline [PBS]) were then added, and scoring was performed after 2-to 12-h incubation at 4°C. For live-cell assays, adherent, confluent cells in 2.....
Document: Hemagglutination assay. Serial twofold dilutions of 100ϫ concentrated virus and pseudovirus particle stocks were prepared using FBS-free DMEM as diluent. Diluted samples were placed into V-bottom 96-well plates and incubated for 2 h at 4°C. Washed adult human erythrocytes (0.5%) (in phosphate-buffered saline [PBS]) were then added, and scoring was performed after 2-to 12-h incubation at 4°C. For live-cell assays, adherent, confluent cells in 24-or 96-well plates were pretreated with vehicle or neuraminidase (1 U/ml) (catalog no. N2876; Sigma) in FBS-free DMEM for 2 h at 37°C. The cells were rinsed three times with PBS and then used in virus transduction assays.
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