Author: Kolodziejek, Jolanta; Seidel, Bernhard; Jungbauer, Christof; Dimmel, Katharina; Kolodziejek, Michael; Rudolf, Ivo; Hubálek, Zdenek; Allerberger, Franz; Nowotny, Norbert
Title: West Nile Virus Positive Blood Donation and Subsequent Entomological Investigation, Austria, 2014 Document date: 2015_5_11
ID: 0vm2hhdr_31
Snippet: For confirmation of the morphologic typing, WNV-positive mosquito samples were investigated by a PCR assay within the mitochondrial 12S rDNA gene recommended for molecular determination of widely divergent arthropods [20] . For the genomic DNA-PCRs a Fast Cycling PCR Kit (Qiagen, USA) was applied. The specificity of PCR products was verified by sequencing, as mentioned above (see section RT-PCR, sequencing and sequence alignment)......
Document: For confirmation of the morphologic typing, WNV-positive mosquito samples were investigated by a PCR assay within the mitochondrial 12S rDNA gene recommended for molecular determination of widely divergent arthropods [20] . For the genomic DNA-PCRs a Fast Cycling PCR Kit (Qiagen, USA) was applied. The specificity of PCR products was verified by sequencing, as mentioned above (see section RT-PCR, sequencing and sequence alignment).
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