Selected article for: "DNA Mini kit and nucleic acid"
Author: Canuti, Marta; Eis-Huebinger, Anna Maria; Deijs, Martin; de Vries, Michel; Drexler, Jan Felix; Oppong, Samuel K.; Müller, Marcel A.; Klose, Stefan M.; Wellinghausen, Nele; Cottontail, Veronika M.; Kalko, Elisabeth K. V.; Drosten, Christian; van der Hoek, Lia
Title: Two Novel Parvoviruses in Frugivorous New and Old World Bats
  • Document date: 2011_12_27
    • ID: 0ewu7e1c_11
    Snippet: To test the organ distribution of the virus and to detect the potential replication site, DNA was also isolated from organ tissues of Eidolon helvum bats found to be parvovirus-positive. Samples collected from brain (n = 7), lungs (n = 7), liver (n = 6), kidneys (n = 7), spleen (n = 7), small intestine (n = 5), and large intestine (n = 4) were analyzed. Nucleic acid was prepared from 20 mg of tissue by using the TissueLyser method with steel bead.....
    Document: To test the organ distribution of the virus and to detect the potential replication site, DNA was also isolated from organ tissues of Eidolon helvum bats found to be parvovirus-positive. Samples collected from brain (n = 7), lungs (n = 7), liver (n = 6), kidneys (n = 7), spleen (n = 7), small intestine (n = 5), and large intestine (n = 4) were analyzed. Nucleic acid was prepared from 20 mg of tissue by using the TissueLyser method with steel beads (Qiagen, Hilden, Germany) and proteinase K in ATL buffer followed by the QIAamp DNA Blood Mini Kit procedure (Qiagen, Hilden, Germany). DNA was eluted in 100 ml.

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