Two Novel Parvoviruses in Frugivorous New and Old World Bats

Canuti, Marta; Eis-Huebinger, Anna Maria; Deijs, Martin; de Vries, Michel; Drexler, Jan Felix; Oppong, Samuel K.; Müller, Marcel A.; Klose, Stefan M.; Wellinghausen, Nele; Cottontail, Veronika M.; Kalko, Elisabeth K. V.; Drosten, Christian; van der Hoek, Lia

Based on phylogenetic analysis of a 740 nt fragment (nucleotides 4329-4561) amplified from different bats, three Aj-BtPV-1 types were distinguishable (Fig. 3) . Types 1 and 2 were the most closely related towards each other (mean between identity of 89.5%, average identity within the types of 95.2% and 95.5%), whereas type 3 (only one sequence available) was the most diverse one (mean between identity of 85.1% and 85.8% vs. types 1 and 2, respectively) (Fig. 3) . This variability was also visible at the amino acid level: 28 out of 246 variable sites could be noted. The virus detected in the bat species Artibeus lituratus belonged to the type 1 cluster. Figure 2 . Phylogenetic relationship of bat parvoviruses with known Parvovirinae. NS1 protein phylogenetic analysis of Eh-BtPV-1 and Aj-BtPV-1 with 59 reference NS1 proteins obtained from GenBank. Genera as well as reference strains' accession numbers are indicated. Members of the Parvovirinae detected in bats are in boldface. The squares indicate the viruses described in this study and the triangle indicates a virus recently identified in bat faeces [16] . doi:10.1371/journal.pone.0029140.g002 Table 2 . Identity ranges (%) of the 3 ORFs both at amino acid (bold) and nucleotide level between species in the PARV4-like viruses a .