Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate

Zapata, Juan Carlos; Carrion, Ricardo; Patterson, Jean L.; Crasta, Oswald; Zhang, Yan; Mani, Sachin; Jett, Marti; Poonia, Bhawna; Djavani, Mahmoud; White, David M.; Lukashevich, Igor S.; Salvato, Maria S.

Gene ontology analysis of global changes in LASV versus ML29-exposed PBMC In order to have a general view of the pathways affected by both virus exposures we performed gene ontology (GO) analysis of differentially expressed genes. The analyses revealed that the most affected genes were involved in type I IFN-stimulated pathways (21%-17 genes from 122), apoptosis (12.29%-15/122), and NFkappa B pathways (10.65%-13/122) ( Figure 2A) . Although, Several genes could be put into more than one category, the interferon-related genes had the higher percentage of presence. From those categories the immune response, defense response and response to viruses were selected for validation analysis ( Table 1 ). The most differentially expressed genes between LASV and ML29 are shown in Table 2 .