Transfer of Anti-Rotavirus Antibodies during Pregnancy and in Milk Following Maternal Vaccination with a Herpes Simplex Virus Type-1 Amplicon Vector

Meier, Anita F.; Suter, Mark; Schraner, Elisabeth M.; Humbel, Bruno M.; Tobler, Kurt; Ackermann, Mathias; Laimbacher, Andrea S.

Vero 2-2 (African green monkey kidney epithelium cells, [42] ), Hepa 1-6 (mouse epithelial hepatocytes, ATCC), MA104 (embryonic African green monkey kidney, ATCC) and HepG2 (human liver hepatocellular cells, ATCC) cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 units/mL of penicillin G, 100 µg/mL of streptomycin, 0.25 µg/mL of amphotericin B and for Vero2-2 cells also with 500 µg/mL of G418 (Thermo Fisher Scientific, Waltham, MA, USA). The murine wild type (wt) RV strain EDIM was obtained from Harry Greenberg (Department of Medicine and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA). The titration of wt RV EDIM used to challenge mice after vaccination was described previously [26] . The human RV strain Wa was obtained from Catherine Eichwald (University of Zurich, Zurich, Switzerland); it was propagated in MA104 cells as previously described [43] .