Author: Zapata, Juan Carlos; Carrion, Ricardo; Patterson, Jean L.; Crasta, Oswald; Zhang, Yan; Mani, Sachin; Jett, Marti; Poonia, Bhawna; Djavani, Mahmoud; White, David M.; Lukashevich, Igor S.; Salvato, Maria S.
Title: Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate Document date: 2013_9_12
ID: 0epeljaf_36
Snippet: To confirm the observed gene expression changes in PBMC exposed to LASV and ML29 viruses, the most affected probesets, the ISG, were selected for further validation. RNA samples from the 24-hr LASV-and ML29-exposed cells were assayed in duplicate using a human IFN and Receptors qRT-PCR Superarray kit (see Methods). Levels of expression of 17 gene-targets were expressed in fold changes (Table 1 and figure 2B )......
Document: To confirm the observed gene expression changes in PBMC exposed to LASV and ML29 viruses, the most affected probesets, the ISG, were selected for further validation. RNA samples from the 24-hr LASV-and ML29-exposed cells were assayed in duplicate using a human IFN and Receptors qRT-PCR Superarray kit (see Methods). Levels of expression of 17 gene-targets were expressed in fold changes (Table 1 and figure 2B ).
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