Selected article for: "electron microscope and transmission electron"
Author: Meier, Anita F.; Suter, Mark; Schraner, Elisabeth M.; Humbel, Bruno M.; Tobler, Kurt; Ackermann, Mathias; Laimbacher, Andrea S.
Title: Transfer of Anti-Rotavirus Antibodies during Pregnancy and in Milk Following Maternal Vaccination with a Herpes Simplex Virus Type-1 Amplicon Vector
  • Document date: 2017_2_16
    • ID: 09hmet7r_56
    Snippet: For negative staining, samples (see section "RVLP purification") were adsorbed to carbon-coated parlodion films mounted on 300 mesh/inch copper grids (Electron Microscopy Sciences (EMS), Fort Washington, PA, USA) for 10 min washed once with H 2 O, and stained with 2% phosphotungstic acid (PTA), pH 7.0 (Aldrich, Steinheim, Germany) for 1 min. Specimens were analyzed in a transmission electron microscope (CM12, Philips, Eindhoven, The Netherlands) .....
    Document: For negative staining, samples (see section "RVLP purification") were adsorbed to carbon-coated parlodion films mounted on 300 mesh/inch copper grids (Electron Microscopy Sciences (EMS), Fort Washington, PA, USA) for 10 min washed once with H 2 O, and stained with 2% phosphotungstic acid (PTA), pH 7.0 (Aldrich, Steinheim, Germany) for 1 min. Specimens were analyzed in a transmission electron microscope (CM12, Philips, Eindhoven, The Netherlands) equipped with a CCD camera (Ultrascan 1000, Gatan, Pleasanton, CA, USA) at an acceleration voltage of 100 kV.

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