Author: Qian, Shaoju; Gao, Zitong; Cao, Rui; Yang, Kang; Cui, Yijie; Li, Shaowen; Meng, Xianrong; He, Qigai; Li, Zili
Title: Transmissible Gastroenteritis Virus Infection Up-Regulates FcRn Expression via Nucleocapsid Protein and Secretion of TGF-ß in Porcine Intestinal Epithelial Cells Document date: 2020_1_21
ID: 06qddkw0_13
Snippet: IPEC-J2 cells at 80% confluence were co-transfected with 0.2 µg FcRn-Luc and 0.1 µg pRL-TK using Lipofectamine 2000 (Invitrogen) reagent before treatment with TGEV, specific siRNA molecules, or recombinant plasmids. Cell lysates were collected at the indicated time points over 36 h and measured using a dual-luciferase assay kit (Promega)......
Document: IPEC-J2 cells at 80% confluence were co-transfected with 0.2 µg FcRn-Luc and 0.1 µg pRL-TK using Lipofectamine 2000 (Invitrogen) reagent before treatment with TGEV, specific siRNA molecules, or recombinant plasmids. Cell lysates were collected at the indicated time points over 36 h and measured using a dual-luciferase assay kit (Promega).
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