Selected article for: "comparative CT method and CT method"
Author: Xing, Yang; Liqi, Zhu; Jian, Lin; Qinghua, Yu; Qian, Yang
Title: Doxycycline Induces Mitophagy and Suppresses Production of Interferon-ß in IPEC-J2 Cells
  • Document date: 2017_2_1
    • ID: 1ljye9pj_17
    Snippet: For quantitative reverse transcription (RT)-PCR (qPCR), total cellular RNA was extracted with TRIZOL (Life Technologies), and RNA was reverse transcribed using the synthesis system (TaKaRa, Dalian, China). qPCR was performed using the Real-Time PCR system (ABI 7500, Life Technologies, USA). Gene expression from three independent experiments was calculated with the comparative Ct method and normalized to the endogenous levels of GAPDH. Primer sequ.....
    Document: For quantitative reverse transcription (RT)-PCR (qPCR), total cellular RNA was extracted with TRIZOL (Life Technologies), and RNA was reverse transcribed using the synthesis system (TaKaRa, Dalian, China). qPCR was performed using the Real-Time PCR system (ABI 7500, Life Technologies, USA). Gene expression from three independent experiments was calculated with the comparative Ct method and normalized to the endogenous levels of GAPDH. Primer sequences used for qPCR are as follows:

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