Author: Wang, Ting; Yin, Huiquan; Li, Yan; Zhao, Lingxiao; Sun, Xiahui; Cong, Hua
Title: Vaccination with recombinant adenovirus expressing multi-stage antigens of Toxoplasma gondii by the mucosal route induces higher systemic cellular and local mucosal immune responses than with other vaccination routes Document date: 2017_4_3
ID: 1ay8y7li_16
Snippet: Spleens were removed from three immunized mice per group four weeks after the last immunization. Single-cell splenocytes were harvested following the method described in a previous study [5, 42] . Isolated splenocytes were plated in 96-well plates, at a density of 1 · 10 6 per well, in 100 lL RPMI-1640 medium (Sigma-Aldrich, USA) supplemented with 10% fetal calf serum and cultured with Concanavalin A (ConA, 2 lg/mL, Sigma-Aldrich) or the UMAS pe.....
Document: Spleens were removed from three immunized mice per group four weeks after the last immunization. Single-cell splenocytes were harvested following the method described in a previous study [5, 42] . Isolated splenocytes were plated in 96-well plates, at a density of 1 · 10 6 per well, in 100 lL RPMI-1640 medium (Sigma-Aldrich, USA) supplemented with 10% fetal calf serum and cultured with Concanavalin A (ConA, 2 lg/mL, Sigma-Aldrich) or the UMAS peptide pool (10 lg/mL). Cell proliferative activity was measured according to the manufacturer's instructions on a Dojindo Cell Counting Kit-8 (Dojindo, Japan). The results were expressed as absorbance at 450 nm.
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