Selected article for: "fecal sample and PCR result"
Author: Greer, Amy L.; Spence, Kelsey; Gardner, Emma
Title: Understanding the early dynamics of the 2014 porcine epidemic diarrhea virus (PEDV) outbreak in Ontario using the incidence decay and exponential adjustment (IDEA) model
  • Document date: 2017_1_5
    • ID: 1g2ij37f_7
    Snippet: Farm data were obtained from publically available reports published by the Ontario Ministry of Agriculture, Food, and Rural Affairs (OMAFRA) documenting all laboratory confirmed porcine epidemic diarrhea virus (PEDV) in the province of Ontario between January 2014 and January 2015 [23] . Ontario disease surveillance guidelines for PEDV requested that veterinarians visiting swine farms where animals showed signs of diarrhea submit samples to the U.....
    Document: Farm data were obtained from publically available reports published by the Ontario Ministry of Agriculture, Food, and Rural Affairs (OMAFRA) documenting all laboratory confirmed porcine epidemic diarrhea virus (PEDV) in the province of Ontario between January 2014 and January 2015 [23] . Ontario disease surveillance guidelines for PEDV requested that veterinarians visiting swine farms where animals showed signs of diarrhea submit samples to the University of Guelph, Animal Health Laboratory (AHL) for testing. Veterinarians were asked to submit a minimum of three pooled fecal samples (with each pooled sample containing feces from 5 different pigs from different litters or locations). Laboratory confirmation was based on a positive test result from the triplex porcine coronavirus polymerase chain reaction (PCR) test. Testing of diagnostic samples was completed at no cost to clients as part of the OMAFRA/ AHL disease surveillance plan. Farms with laboratory confirmed PEDV were encouraged to undertake enhanced biosecurity measures in order minimize the risk to other farms. In addition, herd closures combined with feedback measures to rapidly expose the entire herd and establish herd immunity as quickly as possible were an important component of the response plans. Truck wash protocols (involving sanitation, disinfection, drying and downtime), and manure pumping protocols were put into place in order to minimize the risk of PEDV spread between facilities.

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