Vaccination with recombinant adenovirus expressing multi-stage antigens of Toxoplasma gondii by the mucosal route induces higher systemic cellular and local mucosal immune responses than with other vaccination routes

Wang, Ting; Yin, Huiquan; Li, Yan; Zhao, Lingxiao; Sun, Xiahui; Cong, Hua

Mucosal vaccines against Toxoplasma infection require the induction of protective responses at mucosal surfaces and also the induction of systemic protective immune responses in the systemic compartment [18] . As the first line of defense in protecting mucous membranes, specific SIgA plays a protective role against many pathogens. Therefore, vaccines capable of inducing strong local mucosal immunity represented by secretory IgA would be helpful in preventing T. gondii infection [9] . Our data indicated that T. gondii-specific IgA antibody responses in vaccinated mouse sera were elicited by i.n. and i.o vaccination of Ad-UMAS, which were significantly higher than those induced by other vaccination routes, confirming the ability of Ad-UMAS to induce strong mucosal immunity via the mucosal route.