Selected article for: "dna sequencing and flow cell"
Author: He, Biao; Li, Zuosheng; Yang, Fanli; Zheng, Junfeng; Feng, Ye; Guo, Huancheng; Li, Yingying; Wang, Yiyin; Su, Nan; Zhang, Fuqiang; Fan, Quanshui; Tu, Changchun
Title: Virome Profiling of Bats from Myanmar by Metagenomic Analysis of Tissue Samples Reveals More Novel Mammalian Viruses
  • Document date: 2013_4_22
    • ID: 04d0koah_13
    Snippet: The above purified PCR products of the four groups were pooled together and then subjected to Solexa sequencing in one lane by the Beijing Genome Institute (BGI, Shenzhen, China). Briefly, the pooled purified PCR products were ultrasonicated to produce DNA fragments of about 180 bp, and then treated with Klenow and dATP to generate 39-dA overhangs. After ligation of the fragments to Solexa adaptors, the DNAs were subjected to PCR with the adaptor.....
    Document: The above purified PCR products of the four groups were pooled together and then subjected to Solexa sequencing in one lane by the Beijing Genome Institute (BGI, Shenzhen, China). Briefly, the pooled purified PCR products were ultrasonicated to produce DNA fragments of about 180 bp, and then treated with Klenow and dATP to generate 39-dA overhangs. After ligation of the fragments to Solexa adaptors, the DNAs were subjected to PCR with the adaptor primers to construct a genomic DNA library. The amplicons were bound to a flow cell to which was then added fluorescent-labeled dNTPs. The DNA sequences were obtained by the mechanism of Sequencing-by-Synthesis (SBS; Illumina). Base calling was conducted with the program of GAPipeline using default settings. After removing the adaptor sequences and no-calling reads, the sequences of the four groups were differentiated by their barcodes and then assembled into contigs with SOAPdenove software (BGI, Shenzhen, China). Contigs and sequences longer than 100 bp were defined as significant data for further in silico analysis.

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