Azithromycin and ciprofloxacin have a chloroquine-like effect on respiratory epithelial cells

Vojo Deretic; Graham S Timmins

. CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.29.008631 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.29.008631 doi: bioRxiv preprint E: top row, untreated primary bronchial CF cell; bottom row, primary bronchial CF cell treated with AZT. Color look-up table, R (410/470) ratio of emission intensity at 508 nm upon illumination at 410 and 470 nm. C, TGN pH in IB3-1 (CF) cells with or without treatment with AZT, or in S9 (CFTR-corrected IB3-1 cells). D, correction of TGN pH in primary CF respiratory epithelial cells by AZT. E, ratiometric fluorescence images with cellubrevin-GFP pHluorin in primary CF respiratory epithelial cells from lung transplant. F, correction with AZT of cellubrein endosome pH in CF cells (IB3-1) . G. Correction with AZT of pH in the cellubrevin endosomes in primary CF respiratory epithelial cells, lung transplant, P (ANOVA), * <0.05; ** <0.01, n=6.