Author: Kolodziejek, Jolanta; Seidel, Bernhard; Jungbauer, Christof; Dimmel, Katharina; Kolodziejek, Michael; Rudolf, Ivo; Hubálek, Zdenek; Allerberger, Franz; Nowotny, Norbert
Title: West Nile Virus Positive Blood Donation and Subsequent Entomological Investigation, Austria, 2014 Document date: 2015_5_11
ID: 0vm2hhdr_36
Snippet: The positive WNV-RNA results of this sample were confirmed by the two RT-qPCR assays described above, revealing WNV lineage 2. In addition WNV from the original plasma sample of the blood donor was successfully isolated in suckling mice: one of five mice died on day 6 post inoculation (p.i.). The brain suspension of the dead mouse SMB 1 was further passaged. In the next passage all of the 11 inoculated mice died on days 3 and 4 p.i. with neurolog.....
Document: The positive WNV-RNA results of this sample were confirmed by the two RT-qPCR assays described above, revealing WNV lineage 2. In addition WNV from the original plasma sample of the blood donor was successfully isolated in suckling mice: one of five mice died on day 6 post inoculation (p.i.). The brain suspension of the dead mouse SMB 1 was further passaged. In the next passage all of the 11 inoculated mice died on days 3 and 4 p.i. with neurological symptoms.
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