Author: Romagosa, Anna; Allerson, Matt; Gramer, Marie; Joo, Han Soo; Deen, John; Detmer, Susan; Torremorell, Montserrat
Title: Vaccination of influenza a virus decreases transmission rates in pigs Document date: 2011_12_20
ID: 0q8fedqf_23
Snippet: Nasal swabs were collected daily from individual pigs using rayon-tipped swab applicators with Stuart's medium (BBL CultureSwabâ„¢ liquid, Stuart single plastic applicator/Becton, Dickinson and Com., Sparks, Maryland 21152, USA). After sample collection, each nasal swab was suspended in 2 mL of minimum essential medium (MEM, Mediatech Inc., Manassas, VA, USA) supplemented with 4% bovine serum albumin (BSA, Sigma-Aldrich Co., St. Louis, MO, USA) p.....
Document: Nasal swabs were collected daily from individual pigs using rayon-tipped swab applicators with Stuart's medium (BBL CultureSwabâ„¢ liquid, Stuart single plastic applicator/Becton, Dickinson and Com., Sparks, Maryland 21152, USA). After sample collection, each nasal swab was suspended in 2 mL of minimum essential medium (MEM, Mediatech Inc., Manassas, VA, USA) supplemented with 4% bovine serum albumin (BSA, Sigma-Aldrich Co., St. Louis, MO, USA) prior to processing for RT-PCR. The viral RNA was extracted using the magnetic particle processor procedure (MagMAXâ„¢ Viral RNA Isolation Kit, Applied Biosystems, USA) and subsequently tested using the procedure provided by the USDA-NVSL for detection of influenza A virus Matrix gene by RT-PCR [38] . The minimum detection limit of influenza virus by this method is 10 1 TCID 50 /mL.
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