Vaccination with recombinant adenovirus expressing multi-stage antigens of Toxoplasma gondii by the mucosal route induces higher systemic cellular and local mucosal immune responses than with other vaccination routes

Wang, Ting; Yin, Huiquan; Li, Yan; Zhao, Lingxiao; Sun, Xiahui; Cong, Hua

In summary, mucosal immunization with recombinant adenovirus vaccine expressing ubiquitin-conjugated multistage antigen segments (Ad-UMAS) is a potential vaccination route to elicit protective immune responses. Compared to other immunization routes, oral and nasal immunizations with Ad-UMAS elicit both robust systemic and mucosal immune responses, accompanied by a significant increase in survival rates after challenge with highly virulent parasites and a dramatic reduction in brain cyst burdens in vaccinated mice after challenge with mild strains of T. gondii. Therefore, the results of this study should contribute to the development of an effective and safe mucosal vaccine for preventing T. gondii infection.