Vaccination with recombinant adenovirus expressing multi-stage antigens of Toxoplasma gondii by the mucosal route induces higher systemic cellular and local mucosal immune responses than with other vaccination routes

Wang, Ting; Yin, Huiquan; Li, Yan; Zhao, Lingxiao; Sun, Xiahui; Cong, Hua

Moreover, evaluation of serum IgG subtype antibody responses revealed that all vaccination pathways induced a relatively higher level of Th1-associated IgG2a than Th2associated IgG1 in this study. It should be noted that Ad-UMAS induced a slightly biased Th1-associated antibody (A) (B) Figure 3 . Evaluation of the protective ability against T. gondii infection. (A) Six immunized mice per group were infected intraperitoneally with 1 · 10 3 tachyzoites of T. gondii RH strain 4 weeks after the last immunization. Survival was monitored daily for 28 days after challenge. (B) Another six immunized mice per group were infected intragastrically with 20 cysts of T. gondii PRU strain and the cyst burden in the brain of vaccinated mice was counted 4 weeks later. The mean number of cysts in every mouse group was based on each mouse brain cyst in the group. Asterisks mark the significant difference: *p < 0.05; **p < 0.05. Each bar represents the mean OD (± SD).