Virome Profiling of Bats from Myanmar by Metagenomic Analysis of Tissue Samples Reveals More Novel Mammalian Viruses

He, Biao; Li, Zuosheng; Yang, Fanli; Zheng, Junfeng; Feng, Ye; Guo, Huancheng; Li, Yingying; Wang, Yiyin; Su, Nan; Zhang, Fuqiang; Fan, Quanshui; Tu, Changchun

As shown in table 3, the contigs of vertebrate viruses comprised the largest virus population in the virome of all four groups, and could be classified into 14 families: Adenoviridae, Alloherpesviridae, Herpesviridae, Papillomaviridae, Polyomaviridae, Poxviridae, Picobirnaviridae, Hepadnaviridae, Retroviridae, Circoviridae, Parvoviridae (Parvovirinae), Astroviridae, Flaviviridae and Picornaviridae. Members of the Alloherpesviridae, Herpesviridae, Hepadnaviridae, Papillomaviridae, Parvovirinae, Polyomaviridae, Poxviridae and Picornaviridae were likely common viruses harbored by bats since at least 3 of the 4 groups contained their sequences in viral contigs. These contigs relating to mastadenovirus, circovirus, dependovirus, bocavirus, parvovirus, mamastrovirus, kobuvirus, iflavirus, had strong BLAST bit to viruses with bit score .200 and e value ,10e220. Notably, there were .10 thousand contigs relating to Hepadnaviridae, which covered 43% full genome and shared .70% nt identity with woodchuck hepatitis virus (WHV) and human hepatitis B virus (HBV). Herpesvirus-like contigs had diverse nt identities to known viruses, the longest reads with 210 nt sharing the highest nt identity of 70% with human herpesvirus 6B (data not shown). In the present study, ictalurivirus-, poxvirus-, polyomavirus-, papillomavirus-and hepacivirus-like sequences were not only annotated to viruses but also share nt/aa identities with some cellular organisms, probably as a result of integration of viral genes into their hosts or host genes into viral genomes since large DNA viruses have ability to acquire cellular genes over the course of their evolution [42] [43] [44] [45] . Therefore, to confirm the presence of above large DNA viruses PCR detection and sequencing of longer fragments at multiple viral genomic regions is needed in the future.