Selected article for: "reaction mixture and taq polymerase"
Author: He, Biao; Li, Zuosheng; Yang, Fanli; Zheng, Junfeng; Feng, Ye; Guo, Huancheng; Li, Yingying; Wang, Yiyin; Su, Nan; Zhang, Fuqiang; Fan, Quanshui; Tu, Changchun
Title: Virome Profiling of Bats from Myanmar by Metagenomic Analysis of Tissue Samples Reveals More Novel Mammalian Viruses
  • Document date: 2013_4_22
    • ID: 04d0koah_11
    Snippet: To obtain sufficient viral nucleic acid, SISPA was employed to amplify the dscDNA with the Accuprime Taq DNA Polymerase System (Invitrogen) according to the manufacturer's protocol. Briefly, a 50 ml reaction system containing 10 ml of the above dscDNA mixture, barcode DNA as primer (20 mM), 106Accuprime buffer I, Accuprime Taq DNA Polymerase (1 U) and ddH 2 O was denatured at 94uC for 2 min, followed by 40 cycles of 94uC denaturing for 30 s, 55uC.....
    Document: To obtain sufficient viral nucleic acid, SISPA was employed to amplify the dscDNA with the Accuprime Taq DNA Polymerase System (Invitrogen) according to the manufacturer's protocol. Briefly, a 50 ml reaction system containing 10 ml of the above dscDNA mixture, barcode DNA as primer (20 mM), 106Accuprime buffer I, Accuprime Taq DNA Polymerase (1 U) and ddH 2 O was denatured at 94uC for 2 min, followed by 40 cycles of 94uC denaturing for 30 s, 55uC annealing for 30 s, 68uC extending for 1 min with final 68uC extension for 8 min. The PCR products were then purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) and dissolved in 50 ml TE buffer (100 mM Tris-HCl, 10 mM EDTA, pH8.0).

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