Author: Kolodziejek, Jolanta; Seidel, Bernhard; Jungbauer, Christof; Dimmel, Katharina; Kolodziejek, Michael; Rudolf, Ivo; Hubálek, Zdenek; Allerberger, Franz; Nowotny, Norbert
Title: West Nile Virus Positive Blood Donation and Subsequent Entomological Investigation, Austria, 2014 Document date: 2015_5_11
ID: 0vm2hhdr_8
Snippet: Human WNV positive plasma sample WNV-RNA positive plasma of the blood donor was provided by the Blood Service for Vienna, Lower Austria and Burgenland of the Austrian Red Cross. For PCR, pathogens in the sample were inactivated by immediately adding DNA/RNA Shield solution (Zymo Research, Irvine, USA) in the proportion 1:4 as described by [8] , and stored at -20°C until further processing. The original plasma sample was independently investigate.....
Document: Human WNV positive plasma sample WNV-RNA positive plasma of the blood donor was provided by the Blood Service for Vienna, Lower Austria and Burgenland of the Austrian Red Cross. For PCR, pathogens in the sample were inactivated by immediately adding DNA/RNA Shield solution (Zymo Research, Irvine, USA) in the proportion 1:4 as described by [8] , and stored at -20°C until further processing. The original plasma sample was independently investigated in the Czech laboratory for the presence of neutralizing antibodies against WNV strain Eg-101 by plaque-reduction neutralization test (PRNT) as described previously [9] and it was also used for virus isolation attempts (see below section "Virus isolation").
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