Virome Profiling of Bats from Myanmar by Metagenomic Analysis of Tissue Samples Reveals More Novel Mammalian Viruses

He, Biao; Li, Zuosheng; Yang, Fanli; Zheng, Junfeng; Feng, Ye; Guo, Huancheng; Li, Yingying; Wang, Yiyin; Su, Nan; Zhang, Fuqiang; Fan, Quanshui; Tu, Changchun

There are currently the only two members in the genus Bocavirus and share 43% sequence identity [47, 66] . Animal bocaviruses were first identified in the 1960s, and can cause respiratory, gastrointestinal and reproductive diseases [66] . Human bocavirus was first reported in Sweden in 2005, then in China, 2006, and has been associated with upper and lower respiratory infection and gastroenteritis worldwide [67] [68] [69] . Wu et al. first reported their presence in M. myotis in 2012 [10] . Our study expands their range to include M. fuliginosus in Myanmar. Sequence analysis showed that the Myanmar isolate had about 53% aa identity with canine minute virus (the highest identity) and only 43% identity with Myotis myotis bocavirus. Full genome alignments also revealed their divergences. According to the ICTV criterion that a bocavirus with ,95% nt identity in its NS gene can be defined as a new species, Bt BoV XM30 and WM40 likely form a new species within the genus Bocavirus [48] .