Effects of Storage Time on Total Protein and Globulin Concentrations in Bovine Fresh Frozen Plasma Obtained for Transfusion

Proverbio, D.; Spada, E.; Baggiani, L.; Bagnagatti De Giorgi, G.; Roggero, N.; Belloli, A.; Pravettoni, D.; Perego, R.

Protein fractions were analyzed using a semiautomatic agarose gel electrophoresis (AGE) system (Hydrasys, Sebia PN 1210, Issy-les-Moulineaux, France) (previously validated for bovine). Plasma was electrophoresed for 7 minutes at 33volt hours and stained with diluted Amidoschwarz dye at pH 2 (4 g/L Amidoschwarz dye and 6.7% ethylene glycol). The AGE procedure was performed according to the manufacturer's instructions, and commercial human serum was used as the control (normal control serum, Sebia, Evry, France). Using the computer software Phoresis for Windows 2000 or XP Pro (Sebia), the electrophoretic curve for each sample was displayed. Protein fractions were determined based on the different percentage of optical absorbance and the absolute concentration in g/dL was automatically calculated from the total serum protein concentration. The same operator analyzed all samples. Albumin to globulin (A/G) ratios were also calculated. The percentage of variation observed between mean values of proteins analyzed in fresh and frozen/thawed sera was calculated for each protein fraction.