Selected article for: "10Î cpsÎ and cytolyctic activity"
Author: Meng, Fandan; Wu, Nai-Huei; Seitz, Maren; Herrler, Georg; Valentin-Weigand, Peter
Title: Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions
  • Document date: 2016_5_27
    • ID: 0jsc81sy_6
    Snippet: Results of LDH release and TEER determinations suggest that PBEC are more susceptible to cytotoxic effects of S. suis. Therefore, in the following experiments we focused on the effects of S. suis on PBEC. As expected, the two suilysin-deficient mutants 10Δ sly, 10Δ cpsΔ sly had no detectable cytotoxic effect on PBEC, even at 48 hpi (Fig. 2B,C) . Notably, of the complemented mutant strains expressing point-mutated suilysin cW461F and cS148, res.....
    Document: Results of LDH release and TEER determinations suggest that PBEC are more susceptible to cytotoxic effects of S. suis. Therefore, in the following experiments we focused on the effects of S. suis on PBEC. As expected, the two suilysin-deficient mutants 10Δ sly, 10Δ cpsΔ sly had no detectable cytotoxic effect on PBEC, even at 48 hpi (Fig. 2B,C) . Notably, of the complemented mutant strains expressing point-mutated suilysin cW461F and cS148, respectively, the former showed no cytotoxic effects, whereas the latter induced a significant LDH release at both 4 and 48 hpi (Fig. 2B,C) . Thus, cytotoxic effects on differentiated airway epithelial cells were dependent on the presence of suilysin and required cytolyctic activity, i.e. macropore-formation, of the toxin.

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