Author: Wodrich, Harald; Henaff, Daniel; Jammart, Baptist; Segura-Morales, Carolina; Seelmeir, Sigrid; Coux, Olivier; Ruzsics, Zsolt; Wiethoff, Christopher M.; Kremer, Eric J.
Title: A Capsid-Encoded PPxY-Motif Facilitates Adenovirus Entry Document date: 2010_3_19
ID: 1mjmttec_36
Snippet: A hallmark of Ad5 infection is its transient accumulation at the MTOC during entry [5] . The mutation of the PPxY in the Ad5-VI-M1 virus seemed to alter this localization (Figure 3) . Similarly, the PPxY motif was required for Nedd4.2 dependent ubiquitylation of protein VI. Because knockdown of Nedd4 ligases also diminished transduction with AdGFP vectors we examined accumulation at the MTOC region in cells depleted with control shRNA and Nedd4.1.....
Document: A hallmark of Ad5 infection is its transient accumulation at the MTOC during entry [5] . The mutation of the PPxY in the Ad5-VI-M1 virus seemed to alter this localization (Figure 3) . Similarly, the PPxY motif was required for Nedd4.2 dependent ubiquitylation of protein VI. Because knockdown of Nedd4 ligases also diminished transduction with AdGFP vectors we examined accumulation at the MTOC region in cells depleted with control shRNA and Nedd4.1 and Nedd4.2 specific shRNAs. We used the same strategy as in Figure 3 by quantifying viral particles in proximity to the MTOC, which was identified by stain for pericentrin. MTOC accumulation for Nedd4.1 and Nedd4.2 shRNA treated cells was reduced when compared to cells treated with control shRNA indicating that both ligases might be involved in proper targeting of viruses towards the MTOC ( Figure 8D ).
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