Selected article for: "comparative analysis and reference gene"
Author: Cheung, Benny KW; Yim, Howard CH; Lee, Norris CM; Lau, Allan SY
Title: A novel anti-mycobacterial function of mitogen-activated protein kinase phosphatase-1
  • Document date: 2009_12_17
    • ID: 134qz0aw_39
    Snippet: To perform QPCR, the levels of MKP-1, and TNF-α mRNA as well as the reference gene GAPDH (as internal control) were assayed by the gene-specific Assays-on-Demand reagent kits (Applied Biosystems, USA). All samples were run in duplicates or triplicates and with no template controls on an ABI Prism 7700 Sequence Detector. The analysis method of QPCR was the comparative cycle number to threshold (C T ) method as described in user bulletin no. 2 of .....
    Document: To perform QPCR, the levels of MKP-1, and TNF-α mRNA as well as the reference gene GAPDH (as internal control) were assayed by the gene-specific Assays-on-Demand reagent kits (Applied Biosystems, USA). All samples were run in duplicates or triplicates and with no template controls on an ABI Prism 7700 Sequence Detector. The analysis method of QPCR was the comparative cycle number to threshold (C T ) method as described in user bulletin no. 2 of the ABI Prism 7700 Sequence Detection System. The number of C T of the targeted genes was normalized to that of GAPDH in each sample (ΔC T ). The C T value of the treated cells was compared with that of the untreated or mock-treated cells (ΔΔCT). The relative gene expression of the targeted genes (fold induction) was calculated as 2 -ΔΔCT .

    Search related documents:
    Co phrase search for related documents
    • cycle number and target gene: 1, 2
    • fold induction and target gene: 1
    • gene expression and target gene: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • gene expression and target gene gene expression: 1, 2, 3, 4, 5
    • gene expression and template control: 1, 2, 3
    • gene expression and treat cell: 1, 2, 3
    • internal control and target gene: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
    • internal control and target gene gene expression: 1
    • internal control and template control: 1, 2, 3, 4, 5, 6
    • target gene and template control: 1