Selected article for: "growth medium and humidified incubator"
Author: Griffiths, Samantha J.; Koegl, Manfred; Boutell, Chris; Zenner, Helen L.; Crump, Colin M.; Pica, Francesca; Gonzalez, Orland; Friedel, Caroline C.; Barry, Gerald; Martin, Kim; Craigon, Marie H.; Chen, Rui; Kaza, Lakshmi N.; Fossum, Even; Fazakerley, John K.; Efstathiou, Stacey; Volpi, Antonio; Zimmer, Ralf; Ghazal, Peter; Haas, Jürgen
Title: A Systematic Analysis of Host Factors Reveals a Med23-Interferon-? Regulatory Axis against Herpes Simplex Virus Type 1 Replication
  • Document date: 2013_8_8
    • ID: 0lyt8gfq_53
    Snippet: Selected siRNAs SMARTpools were diluted to 500 nM in HBSS and 40 ml was incubated with 40 ml Dharmafect 1 diluted in HBSS to a final concentration of 0.15%. After 20 min incubation, 3610 4 Hela cells in 320 ml transfection medium were added, mixed with the transfection complexes and transferred to 8-well glass bottomed chamber slides (Becton Dickinson). Plates were incubated for 48 h at 37uC in a humidified incubator with 5% CO 2 before infection.....
    Document: Selected siRNAs SMARTpools were diluted to 500 nM in HBSS and 40 ml was incubated with 40 ml Dharmafect 1 diluted in HBSS to a final concentration of 0.15%. After 20 min incubation, 3610 4 Hela cells in 320 ml transfection medium were added, mixed with the transfection complexes and transferred to 8-well glass bottomed chamber slides (Becton Dickinson). Plates were incubated for 48 h at 37uC in a humidified incubator with 5% CO 2 before infection by removing medium and adding 100 ml HSV-1-eGFP at a MOI of 1. After incubation for 1 h at 37uC, virus was removed and replaced with 500 ml growth medium. Images were acquired 48 h post-infection.

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