Author: Joshua A. Hayward; Mary Tachedjian; Adam Johnson; Tamsin B. Gordon; Jie Cui; Glenn A. Marsh; Michelle L. Baker; Lin-Fa Wang; Gilda Tachedjian
Title: Bats Possess Unique Variants of the Antiviral Restriction Factor Tetherin Document date: 2020_4_9
ID: eksi9yia_27
Snippet: All tetherin coding sequences were modified with the addition of a haemagglutinin (HA) tag sequence (N-SGYPYDVPDYAGS-C) to enable antibody-based detection of protein expression. In all cases the HA tag was inserted at the position immediately following the end of the coiled-coil region of the extracellular domain ( Figure 1A ) which is the equivalent location to that which has been previously utilized in the tagging of human tetherin (22) . The c.....
Document: All tetherin coding sequences were modified with the addition of a haemagglutinin (HA) tag sequence (N-SGYPYDVPDYAGS-C) to enable antibody-based detection of protein expression. In all cases the HA tag was inserted at the position immediately following the end of the coiled-coil region of the extracellular domain ( Figure 1A ) which is the equivalent location to that which has been previously utilized in the tagging of human tetherin (22) . The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.031203 doi: bioRxiv preprint
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