Author: Claus, Claudia; Manssen, Lena; Hübner, Denise; Roßmark, Sarah; Bothe, Viktoria; Petzold, Alice; Große, Claudia; Reins, Mareen; Mankertz, Annette; Frey, Teryl K.; Liebert, Uwe G.
Title: Activation of the Mitochondrial Apoptotic Signaling Platform during Rubella Virus Infection Document date: 2015_11_26
ID: 1rrm4sqa_37
Snippet: Taking this high level of nuclear translocation of p53 into account, the mRNA expression level of p21 Waf1/Cip1 (p21) was determined, which is regulated by the transcriptional activity of p53. Despite the intensive nuclear localization of p53 under Wb-12 infection, the induction of p21 mRNA expression is lower than the one observed for Therien ( Figure 8C ). Results presented point to nuclear localization of p53 as a distinguishing feature betwee.....
Document: Taking this high level of nuclear translocation of p53 into account, the mRNA expression level of p21 Waf1/Cip1 (p21) was determined, which is regulated by the transcriptional activity of p53. Despite the intensive nuclear localization of p53 under Wb-12 infection, the induction of p21 mRNA expression is lower than the one observed for Therien ( Figure 8C ). Results presented point to nuclear localization of p53 as a distinguishing feature between RV cell-culture-adapted strains and clinical isolates. Immunofluorescence analysis was performed with anti-p53 antibody to assess intracellular distribution of p53 (shown in red). RV infection was visualized with anti-E1 antibody (shown in green). Nuclear DNA is shown in blue. Pink colour indicates overlap areas. (C) Alteration of the mRNA expression level of p21 after infection with RV strains, Therien and Wb-12. The mRNA expression level was determined at 1, 2 and 3 dpi relative to the corresponding mock sample (set at 100%). Scale bar, 20 μm. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Search related documents:
Co phrase search for related documents- anti e1 antibody and clinical isolate: 1
- anti e1 antibody and expression level: 1
- anti e1 antibody and immunofluorescence analysis: 1, 2, 3, 4
- anti e1 antibody and intracellular distribution: 1, 2, 3
- anti e1 antibody and mock sample: 1
- anti e1 antibody and mrna expression level: 1
- anti e1 antibody and nuclear localization: 1
- anti e1 antibody and nuclear translocation: 1, 2
- blue show and green show: 1
- cell culture and clinical isolate: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
- cell culture and expression level: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
- cell culture and high level: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell culture and immunofluorescence analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- cell culture and intracellular distribution: 1, 2, 3, 4, 5
- cell culture and mock sample: 1, 2
- cell culture and mrna expression level: 1, 2, 3
- cell culture and nuclear localization: 1, 2, 3, 4, 5
- cell culture and nuclear translocation: 1, 2, 3, 4, 5, 6, 7
- clinical isolate and nuclear localization: 1, 2
Co phrase search for related documents, hyperlinks ordered by date