Selected article for: "chimeric protein and replacement solution"

Author: Jones, Harrison G.; Battles, Michael B.; Lin, Chun-Chi; Bianchi, Siro; Corti, Davide; McLellan, Jason S.
Title: Alternative conformations of a major antigenic site on RSV F
  • Document date: 2019_7_15
  • ID: 1r20hl2b_38
    Snippet: Diffraction data were processed using the CCP4 software suite [65] : data were indexed and integrated in iMOSFLM [66] and scaled and merged with AIMLESS [67] . A molecular replacement solution for the 1.70 Ã… AM22 Fab dataset was found by PHASER [68] using a chimeric protein model consisting of the heavy and light chains of PDB ID: 3LMJ and PDB ID: 3QEG, respectively, separated into the constant and variable domains as search models. The structur.....
    Document: Diffraction data were processed using the CCP4 software suite [65] : data were indexed and integrated in iMOSFLM [66] and scaled and merged with AIMLESS [67] . A molecular replacement solution for the 1.70 Ã… AM22 Fab dataset was found by PHASER [68] using a chimeric protein model consisting of the heavy and light chains of PDB ID: 3LMJ and PDB ID: 3QEG, respectively, separated into the constant and variable domains as search models. The structure was built manually in Coot [69] and refined using PHENIX [70] . The structure was built and refined to an R work /R free of 18.0%/20.5% (Table 1) .

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