Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens Document date: 2016_8_9
ID: 16e99fuz_30
Snippet: mTEC culture. Primary murine tracheal epithelial cells (mTEC) were isolated as previously described [62] . Briefly, tracheas were collected from euthanized mice and epithelial cells collected by scraping. Cells were cultured on transwell inserts pre-coated with 5 μg/cm 2 of both rat-tail collagen type I (BD Biosciences, San Diego, CA) and human fibronectin (BD Biosciences). When cells reached 100% confluency, they were cultured at air-liquid int.....
Document: mTEC culture. Primary murine tracheal epithelial cells (mTEC) were isolated as previously described [62] . Briefly, tracheas were collected from euthanized mice and epithelial cells collected by scraping. Cells were cultured on transwell inserts pre-coated with 5 μg/cm 2 of both rat-tail collagen type I (BD Biosciences, San Diego, CA) and human fibronectin (BD Biosciences). When cells reached 100% confluency, they were cultured at air-liquid interface for 3-4 weeks until fully differentiated (defined as trans-epithelial resistance > 300 O cm 2 ).
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