Selected article for: "confocal microscope and room temperature"
Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens
  • Document date: 2016_8_9
    • ID: 16e99fuz_40
    Snippet: YFP staining. Fixed lung tissue from uninfected WT or β6 KO mice containing the Ifnb1-YFP reporter was cryoprotected with 20% sucrose prior to embedding. 20 μm thick sections were air dried and subsequently treated with cold acetone for 5 min. Following rehydration in PBS, sections were incubated with Alexa555-conjugated wheat germ agglutinin (1μg/ml; Ther-moFisher, W32464) in PBS containing 1% BSA for 30 min at room temperature. Slides were w.....
    Document: YFP staining. Fixed lung tissue from uninfected WT or β6 KO mice containing the Ifnb1-YFP reporter was cryoprotected with 20% sucrose prior to embedding. 20 μm thick sections were air dried and subsequently treated with cold acetone for 5 min. Following rehydration in PBS, sections were incubated with Alexa555-conjugated wheat germ agglutinin (1μg/ml; Ther-moFisher, W32464) in PBS containing 1% BSA for 30 min at room temperature. Slides were washed in PBS, mounted with hardset media (Vector laboratories), and imaged using a Marianis spinning disk confocal microscope (Intelligent Imaging Innovations) equipped with a 63X 1.2NA objective and EMCCD camera, and analyzed using Slidebook software (Intelligent Imaging Innovations). Ten separate fields were imaged per mouse.

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