Selected article for: "CD11b surface and flow cytometry"
Author: Meliopoulos, Victoria A.; Van de Velde, Lee-Ann; Van de Velde, Nicholas C.; Karlsson, Erik A.; Neale, Geoff; Vogel, Peter; Guy, Cliff; Sharma, Shalini; Duan, Susu; Surman, Sherri L.; Jones, Bart G.; Johnson, Michael D. L.; Bosio, Catharine; Jolly, Lisa; Jenkins, R. Gisli; Hurwitz, Julia L.; Rosch, Jason W.; Sheppard, Dean; Thomas, Paul G.; Murray, Peter J.; Schultz-Cherry, Stacey
Title: An Epithelial Integrin Regulates the Amplitude of Protective Lung Interferon Responses against Multiple Respiratory Pathogens
  • Document date: 2016_8_9
    • ID: 16e99fuz_54
    Snippet: Flow cytometry. Lung cells were treated with Fc block and surface stained with anti-CD11b (M1/70, BioLegend) (1 μg/ml), anti-CD11c (HL3, BD Pharmigen) (1 μg/ml), and anti-F4/80 (BM8, BioLegend) (1 μg/ml) antibodies. The alveolar macrophage population was gated on autofluorescence in the FL-1 channel and high F4/80 expression relative to single color controls. In some experiments further gating with SiglecF (E50-2440, BD Pharmagen, 552126) (1 Î.....
    Document: Flow cytometry. Lung cells were treated with Fc block and surface stained with anti-CD11b (M1/70, BioLegend) (1 μg/ml), anti-CD11c (HL3, BD Pharmigen) (1 μg/ml), and anti-F4/80 (BM8, BioLegend) (1 μg/ml) antibodies. The alveolar macrophage population was gated on autofluorescence in the FL-1 channel and high F4/80 expression relative to single color controls. In some experiments further gating with SiglecF (E50-2440, BD Pharmagen, 552126) (1 μg/ml), MerTK (DS5MMER, eBioscience, 12-5751-82) (1:100 dilution), CD64 (X54-5/7.1, BioLegend, 139306) (1:100 dilution), was performed.

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