Selected article for: "antibody production and expression system"
Author: Saeed, Abdullah F. U. H.; Wang, Rongzhi; Ling, Sumei; Wang, Shihua
Title: Antibody Engineering for Pursuing a Healthier Future
  • Document date: 2017_3_28
    • ID: 0fegsm1v_63
    Snippet: Numerous expression systems such as E. coli, yeast, mammalian, insect cell, wheat germ cell-free expression system, and plantbased expression system have been used for the successful isolation of scFv and display as fragments . The expression and activation of scFv is performed by appropriate folding and in vitro refolding for aggregation. The expression systems for the production of active scFv antibody are selected, designed, and constructed ba.....
    Document: Numerous expression systems such as E. coli, yeast, mammalian, insect cell, wheat germ cell-free expression system, and plantbased expression system have been used for the successful isolation of scFv and display as fragments . The expression and activation of scFv is performed by appropriate folding and in vitro refolding for aggregation. The expression systems for the production of active scFv antibody are selected, designed, and constructed based on hosts. The bacterial expression system is the most suitable and widely used method for the production of scFv antibody fragments compared to other available expression strategies (Frenzel et al., 2013) .

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