Author: Lum, Fok-Moon; Couderc, Thérèse; Chia, Bing-Shao; Ong, Ruo-Yan; Her, Zhisheng; Chow, Angela; Leo, Yee-Sin; Kam, Yiu-Wing; Rénia, Laurent; Lecuit, Marc; Ng, Lisa F. P.
Title: Antibody-mediated enhancement aggravates chikungunya virus infection and disease severity Document date: 2018_1_30
ID: 1vhzto1o_27
Snippet: Gene expression. Total RNA from cells was extracted using RNeasy ® Mini Kit (QIAGEN) according to manufacturer's instructions, without any DNAse treatment. Eluted RNA was quantified and diluted to 10 ng/ µl before being stored in −80 °C. Gene expression profiling was performed via RT-PCR using QuantiFAST TM SYBR ® Green RT-PCR Kit (QIAGEN) as described 47 . Results are expressed as relative fold change in expression level compared to mock i.....
Document: Gene expression. Total RNA from cells was extracted using RNeasy ® Mini Kit (QIAGEN) according to manufacturer's instructions, without any DNAse treatment. Eluted RNA was quantified and diluted to 10 ng/ µl before being stored in −80 °C. Gene expression profiling was performed via RT-PCR using QuantiFAST TM SYBR ® Green RT-PCR Kit (QIAGEN) as described 47 . Results are expressed as relative fold change in expression level compared to mock infections, as previously described 47 . Gene expression data from infected RAW264.7 cells are displayed by two-way hierarchical clustering generated with Multi Experiment Viewer (version 4.9) (Microarray Software Suite TM4) 72 . Sequences of primers used are displayed in Table S1 .
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