Author: Lum, Fok-Moon; Couderc, Thérèse; Chia, Bing-Shao; Ong, Ruo-Yan; Her, Zhisheng; Chow, Angela; Leo, Yee-Sin; Kam, Yiu-Wing; Rénia, Laurent; Lecuit, Marc; Ng, Lisa F. P.
Title: Antibody-mediated enhancement aggravates chikungunya virus infection and disease severity Document date: 2018_1_30
ID: 1vhzto1o_31
Snippet: For total RNA extraction from excised joint footpad, specimens were first shredded in TRIzol (Invitrogen) with Micro Smash TM (Digital Biology), a micro-homogenizing system, following these conditions: 2 rounds of homogenization at 5000 rpm for 45 s followed by 30 s on ice. Subsequently, the sample was spun to remove the unwanted cell debris. Chloroform was then added to the TRIzol fraction causing a phase separation into the aqueous and organic .....
Document: For total RNA extraction from excised joint footpad, specimens were first shredded in TRIzol (Invitrogen) with Micro Smash TM (Digital Biology), a micro-homogenizing system, following these conditions: 2 rounds of homogenization at 5000 rpm for 45 s followed by 30 s on ice. Subsequently, the sample was spun to remove the unwanted cell debris. Chloroform was then added to the TRIzol fraction causing a phase separation into the aqueous and organic phase. The aqueous phase, which contains the RNA, was carefully removed into a clean eppendorf tube. It was then further processed with the RNeasy ® Mini Kit (QIAGEN) according to manufacturer's instructions, before being used for gene expression studies as described above. No DNAse treatment was performed during the total RNA extraction.
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