Selected article for: "room temperature and total number"
Author: Gwon, Yong-Dae; Strand, Mårten; Lindqvist, Richard; Nilsson, Emma; Saleeb, Michael; Elofsson, Mikael; Överby, Anna K.; Evander, Magnus
Title: Antiviral Activity of Benzavir-2 against Emerging Flaviviruses
  • Document date: 2020_3_22
    • ID: 0ym40eki_16
    Snippet: After incubation, the medium was removed, and cells were fixed with 4% paraformaldehyde for 1 h at room temperature and washed with phosphate-buffered saline (PBS). The fixed cells were counterstained with 300 nM 4 ,6-diamidino-2-phenylindole (DAPI) in PBS. The Trophos plate runner HD ® (Trophos, Roche Group, Basel, Switzerland) identified all individual ZsGreen-expressing cells or DAPI-stained cells and quantified the fluorescence intensity in .....
    Document: After incubation, the medium was removed, and cells were fixed with 4% paraformaldehyde for 1 h at room temperature and washed with phosphate-buffered saline (PBS). The fixed cells were counterstained with 300 nM 4 ,6-diamidino-2-phenylindole (DAPI) in PBS. The Trophos plate runner HD ® (Trophos, Roche Group, Basel, Switzerland) identified all individual ZsGreen-expressing cells or DAPI-stained cells and quantified the fluorescence intensity in each well. The infection rates were calculated by dividing the number of infected cells (ZsGreen-expressing cells) to the total number of cells (DAPI-stained cells). The EC 50 values were calculated by non-linear regression analysis with a variable slope, using GraphPad Prism 7.0 software. Furthermore, the EC 90 values were calculated by Quick → Calcs, which is provided at GraphPad website [25] 2.6. Cell Toxicity Assay

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