Author: Gwon, Yong-Dae; Strand, Mårten; Lindqvist, Richard; Nilsson, Emma; Saleeb, Michael; Elofsson, Mikael; Överby, Anna K.; Evander, Magnus
Title: Antiviral Activity of Benzavir-2 against Emerging Flaviviruses Document date: 2020_3_22
ID: 0ym40eki_21
Snippet: Vero B4 cells were seeded in 12-well plates (2 × 10 5 cells per well) and wt ZIKV (300 PFU) solution, together with 2.5 µM of benzavir-2 in DMEM, was added, and the cells were incubated for 4 days. The supernatants were collected from infected cells with or without compounds. Next, the collected supernatants were transferred to new Vero B4 cell layers (indicator cells) for 1 h and replaced with DMEM with 1% CMC (for CPE observation and crystal .....
Document: Vero B4 cells were seeded in 12-well plates (2 × 10 5 cells per well) and wt ZIKV (300 PFU) solution, together with 2.5 µM of benzavir-2 in DMEM, was added, and the cells were incubated for 4 days. The supernatants were collected from infected cells with or without compounds. Next, the collected supernatants were transferred to new Vero B4 cell layers (indicator cells) for 1 h and replaced with DMEM with 1% CMC (for CPE observation and crystal violet staining) or with fresh DMEM (for qRT-PCR), respectively. The plates were analyzed by observing virus-induced CPE 2 days post-infection, and plaque formation was determined by crystal violet staining on day 4. The supernatants were collected and further used for viral RNA isolation and quantification of virus RNA in the supernatant.
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