Author: Gwon, Yong-Dae; Strand, Mårten; Lindqvist, Richard; Nilsson, Emma; Saleeb, Michael; Elofsson, Mikael; Överby, Anna K.; Evander, Magnus
Title: Antiviral Activity of Benzavir-2 against Emerging Flaviviruses Document date: 2020_3_22
ID: 0ym40eki_35
Snippet: The host cell toxicity of benzavir-2 and the structural analogs have previously been assessed using other cell types than Vero B4 [16, 18, 19] . As previously reported, solubility issues above 125 µM in cell media have been observed for benzavir-2. Hence, we set the maximum concentration for toxicity assessment to 100 µM for all compounds. The Vero B4 cell toxicity was analyzed 24 h post-treatment using a resazurin-based toxicity test, which is.....
Document: The host cell toxicity of benzavir-2 and the structural analogs have previously been assessed using other cell types than Vero B4 [16, 18, 19] . As previously reported, solubility issues above 125 µM in cell media have been observed for benzavir-2. Hence, we set the maximum concentration for toxicity assessment to 100 µM for all compounds. The Vero B4 cell toxicity was analyzed 24 h post-treatment using a resazurin-based toxicity test, which is a colorimetric assay that measures the metabolic activity of living cells [32] . All compounds displayed cell viability well above 50% at the highest concentration of 100 µM, thus, no CC 50 value could be measured and no selectivity index (SI) could be presented (Table 1) .
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