Author: Liu, Wen-Kuan; Liu, Qian; Chen, De-Hui; Liang, Huan-Xi; Chen, Xiao-Kai; Huang, Wen-Bo; Qin, Sheng; Yang, Zi-Feng; Zhou, Rong
Title: Epidemiology and clinical presentation of the four human parainfluenza virus types Document date: 2013_1_23
ID: 1b56a6zc_8
Snippet: Real-time polymerase chain reaction (PCR) for detection of HPIV and other common respiratory pathogens DNA or RNA from respiratory samples was extracted using QIAamp DNA Mini Kit or QIAamp Viral RNA Mini Kit (Qiagen Co. Ltd., Shanghai, China) in accordance with the manufacturer's protocols. The four types of HPIV were tested by TaqMan real-time PCR as previously reported [19] , and 13 other common respiratory pathogens were also selected for TaqM.....
Document: Real-time polymerase chain reaction (PCR) for detection of HPIV and other common respiratory pathogens DNA or RNA from respiratory samples was extracted using QIAamp DNA Mini Kit or QIAamp Viral RNA Mini Kit (Qiagen Co. Ltd., Shanghai, China) in accordance with the manufacturer's protocols. The four types of HPIV were tested by TaqMan real-time PCR as previously reported [19] , and 13 other common respiratory pathogens were also selected for TaqMan real-time PCR testing, including influenza A virus (infA), influenza B virus (infB), respiratory syncytial virus (RSV), adenovirus (ADV), enterovirus (EV), human metapneumovirus (HMPV), four strains of human coronavirus (HCoV-229E, OC43, NL63 and HKU1), human bocavirus (HBoV), Mycoplasma pneumoniae (MP), and Chlamydophila pneumoniae (CP) [19] .
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