Author: Lu, Qing-Bin; Tong, Yi-Gang; Wo, Ying; Wang, Hong-Yu; Liu, En-Mei; Gray, Gregory C; Liu, Wei; Cao, Wu-Chun
Title: Epidemiology of human adenovirus and molecular characterization of human adenovirus 55 in China, 2009–2012 Document date: 2014_1_28
ID: 0jb8vgt9_6
Snippet: DNA and RNA were extracted from each specimen using the QIAamp â MinElute Virus Spin Kits (QIAGEN, Hilden, Germany). Molecular assays for HAdV were performed using pan primers as previously described. 11 Specimens were screened for human bocavirus (HBoV), and influenza A virus by real-time RT-PCR 12 as well as for respiratory syncytial virus (RSV), parainfluenza virus (PIV), human metapneumovirus (MPV), and coronavirus by nested PCR. 13 Each PCR.....
Document: DNA and RNA were extracted from each specimen using the QIAamp â MinElute Virus Spin Kits (QIAGEN, Hilden, Germany). Molecular assays for HAdV were performed using pan primers as previously described. 11 Specimens were screened for human bocavirus (HBoV), and influenza A virus by real-time RT-PCR 12 as well as for respiratory syncytial virus (RSV), parainfluenza virus (PIV), human metapneumovirus (MPV), and coronavirus by nested PCR. 13 Each PCR run included virus isolates DNA or RNA as positive control and water as negative control.
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