Selected article for: "cell line and genomic dna"

Author: Borkosky, Silvia S.; Whitley, Corinna; Kopp-Schneider, Annette; zur Hausen, Harald; deVilliers, Ethel-Michele
Title: Epstein-Barr Virus Stimulates Torque Teno Virus Replication: A Possible Relationship to Multiple Sclerosis
  • Document date: 2012_2_22
  • ID: 0fl0heq1_28
    Snippet: EBV DNA was quantified by targeting the BALF1 gene [75] in the EBV positive cell lines. Genomic DNA samples (2 ng) in triplicates were used for the reaction and NTC included in each reaction plate. Primers and probes used to detect EBV DNA are listed in Table 1 . Quantification of EBV DNA in each cell line was calculated against a standard curve generated by serial dilutions of Namalwa DNA (2 copies of EBV DNA per cell) as previously described [7.....
    Document: EBV DNA was quantified by targeting the BALF1 gene [75] in the EBV positive cell lines. Genomic DNA samples (2 ng) in triplicates were used for the reaction and NTC included in each reaction plate. Primers and probes used to detect EBV DNA are listed in Table 1 . Quantification of EBV DNA in each cell line was calculated against a standard curve generated by serial dilutions of Namalwa DNA (2 copies of EBV DNA per cell) as previously described [75] .

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