Selected article for: "limit detection and specificity sensitivity"
Author: Fongaro, Gislaine; Nascimento, Mariana A do; Rigotto, Caroline; Ritterbusch, Giseli; da Silva, Alessandra D’ A; Esteves, Paulo A; Barardi, Célia R M
Title: Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays
  • Document date: 2013_5_28
    • ID: 19aj551d_21
    Snippet: All the samples containing particles of infectious HAdV detected by PA were also detected with ICC-RT-qPCR, but the reverse was not true. ICC-RT-qPCR demonstrated greater sensitivity and speed of detection of infectious viral particles compared to PA. ICC-RT-qPCR had a detection limit of 1×10 2 GC/mL at 24 h. Specificity and sensitivity are also important aspects to consider, as the ICC-RT-qPCR relies on mRNA and thus avoids false negatives or p.....
    Document: All the samples containing particles of infectious HAdV detected by PA were also detected with ICC-RT-qPCR, but the reverse was not true. ICC-RT-qPCR demonstrated greater sensitivity and speed of detection of infectious viral particles compared to PA. ICC-RT-qPCR had a detection limit of 1×10 2 GC/mL at 24 h. Specificity and sensitivity are also important aspects to consider, as the ICC-RT-qPCR relies on mRNA and thus avoids false negatives or positives [25, 26] .

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