Author: Zhang, Ying; Cao, Lan; Xu, Zhi; Zhu, Pingting; Huang, Bing; Li, Kuibiao; Xu, Yang; Zhang, Zhoubin; Wu, Yong; Di, Biao
Title: Evaluation of a multiplex PCR assay for detection of respiratory viruses and Mycoplasma pneumoniae in oropharyngeal swab samples from outpatients Document date: 2019_10_19
ID: 07dpy0jn_8
Snippet: The nucleic acid was subjected to multiplex amplification for all specimens using SureX instructions. Each pathogen, if detectable, produced a distinctive fragment size after PCR amplification. The results of fragment analysis were used to determine the outcomes of testing. In brief, if the peak height of a targeted fragment size is lower than the lower peak of the signal standard, the targeted pathogen is determined negative; if the peak height .....
Document: The nucleic acid was subjected to multiplex amplification for all specimens using SureX instructions. Each pathogen, if detectable, produced a distinctive fragment size after PCR amplification. The results of fragment analysis were used to determine the outcomes of testing. In brief, if the peak height of a targeted fragment size is lower than the lower peak of the signal standard, the targeted pathogen is determined negative; if the peak height of a targeted fragment size is higher than the higher peak of the signal standard, the targeted pathogen is determined positive; if the peak height of a targeted fragment size is between the higher and the lower peaks of the signal standard, the targeted pathogen is determined uncertain and the test should be repeated.
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