Author: Drexler, Jan Felix; Corman, Victor Max; Müller, Marcel Alexander; Lukashev, Alexander N.; Gmyl, Anatoly; Coutard, Bruno; Adam, Alexander; Ritz, Daniel; Leijten, Lonneke M.; van Riel, Debby; Kallies, Rene; Klose, Stefan M.; Gloza-Rausch, Florian; Binger, Tabea; Annan, Augustina; Adu-Sarkodie, Yaw; Oppong, Samuel; Bourgarel, Mathieu; Rupp, Daniel; Hoffmann, Bernd; Schlegel, Mathias; Kümmerer, Beate M.; Krüger, Detlev H.; Schmidt-Chanasit, Jonas; Setién, Alvaro Aguilar; Cottontail, Veronika M.; Hemachudha, Thiravat; Wacharapluesadee, Supaporn; Osterrieder, Klaus; Bartenschlager, Ralf; Matthee, Sonja; Beer, Martin; Kuiken, Thijs; Reusken, Chantal; Leroy, Eric M.; Ulrich, Rainer G.; Drosten, Christian
Title: Evidence for Novel Hepaciviruses in Rodents Document date: 2013_6_20
ID: 1v353uij_9
Snippet: For all sampling and exportation of specimens, permission was obtained from the respective authorities (see Acknowledgement for individual permits). Animals were caught with mist nets, live or snap traps, identified by trained field biologists on site or prior to dissection (where applicable), euthanized and dissected in the respective laboratories. Canine, feline and equine samples were routine diagnostic specimens. Between 10-140 mL of blood we.....
Document: For all sampling and exportation of specimens, permission was obtained from the respective authorities (see Acknowledgement for individual permits). Animals were caught with mist nets, live or snap traps, identified by trained field biologists on site or prior to dissection (where applicable), euthanized and dissected in the respective laboratories. Canine, feline and equine samples were routine diagnostic specimens. Between 10-140 mL of blood were extracted using the Qiagen Viral RNA Mini kit (Qiagen, Hilden, Germany). Approximately 30 mg of solid organ tissue were homogenized in a TissueLyser (Qiagen) and purified using the RNeasy Kit (Qiagen).
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