Selected article for: "Î Actin loading control and loading control"
Author: Zhai, Xiaofeng; Wang, Shilei; Zhu, Mengyan; He, Wei; Pan, Zhongzhou; Su, Shuo
Title: Antiviral Effect of Lithium Chloride and Diammonium Glycyrrhizinate on Porcine Deltacoronavirus In Vitro
  • Document date: 2019_9_9
    • ID: 1m5yr8uc_13
    Snippet: Total cellular RNA was extracted using the RNApure Tissue & Cell Kit (CWBIO, Nanjing, China). The primers for PDCoV M and β-actin genes were: PDCoV-F, 5 -ACAATCGACCACATGGCTCCAA-3 , PDCoV-R, 5 -CAGCTCTTGCCCATGTAGCTTCA-3 , β-actin-F, 5 -CTCCATCATGAAGTGCGACGT-3 , β-actin-R, 5 -GTGATCTCCTTCTGCATCCTGTC-3 [1] . The quantity of vRNA was determined using a LightCycler96 (Roche, Germany) and HiScript II One Step qRT-PCR SYBR Green Kit (Vazyme, Nanjing,.....
    Document: Total cellular RNA was extracted using the RNApure Tissue & Cell Kit (CWBIO, Nanjing, China). The primers for PDCoV M and β-actin genes were: PDCoV-F, 5 -ACAATCGACCACATGGCTCCAA-3 , PDCoV-R, 5 -CAGCTCTTGCCCATGTAGCTTCA-3 , β-actin-F, 5 -CTCCATCATGAAGTGCGACGT-3 , β-actin-R, 5 -GTGATCTCCTTCTGCATCCTGTC-3 [1] . The quantity of vRNA was determined using a LightCycler96 (Roche, Germany) and HiScript II One Step qRT-PCR SYBR Green Kit (Vazyme, Nanjing, China), according to the instructions of the manufacturer. The relative level of RNA expression was determined by the 2 −∆∆CT method. β-actin mRNA level was used as a loading control. The mean RNA level of the mock-treated group was set at 100%.

    Search related documents:
    Co phrase search for related documents
    • Cell kit and mRNA level: 1, 2, 3
    • Cell kit and qrt pcr: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
    • Cell kit and RNA expression: 1, 2, 3, 4
    • Green kit and β actin gene: 1, 2, 3
    • Green kit and loading control: 1
    • Green kit and qrt pcr: 1, 2, 3
    • Green kit and relative level: 1
    • Green kit and RNA expression: 1
    • β actin gene and loading control: 1
    • β actin gene and qrt pcr: 1, 2, 3, 4, 5, 6, 7
    • loading control and qrt pcr: 1, 2, 3, 4, 5
    • loading control and RNA expression: 1, 2
    • mRNA level and qrt pcr: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
    • mRNA level and relative level: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • mRNA level and RNA expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
    • mRNA level and RNA expression relative level: 1
    • qrt pcr and RNA expression: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25