Selected article for: "actin mrna and loading control"
Author: Zhai, Xiaofeng; Wang, Shilei; Zhu, Mengyan; He, Wei; Pan, Zhongzhou; Su, Shuo
Title: Antiviral Effect of Lithium Chloride and Diammonium Glycyrrhizinate on Porcine Deltacoronavirus In Vitro
  • Document date: 2019_9_9
    • ID: 1m5yr8uc_13
    Snippet: Total cellular RNA was extracted using the RNApure Tissue & Cell Kit (CWBIO, Nanjing, China). The primers for PDCoV M and β-actin genes were: PDCoV-F, 5 -ACAATCGACCACATGGCTCCAA-3 , PDCoV-R, 5 -CAGCTCTTGCCCATGTAGCTTCA-3 , β-actin-F, 5 -CTCCATCATGAAGTGCGACGT-3 , β-actin-R, 5 -GTGATCTCCTTCTGCATCCTGTC-3 [1] . The quantity of vRNA was determined using a LightCycler96 (Roche, Germany) and HiScript II One Step qRT-PCR SYBR Green Kit (Vazyme, Nanjing,.....
    Document: Total cellular RNA was extracted using the RNApure Tissue & Cell Kit (CWBIO, Nanjing, China). The primers for PDCoV M and β-actin genes were: PDCoV-F, 5 -ACAATCGACCACATGGCTCCAA-3 , PDCoV-R, 5 -CAGCTCTTGCCCATGTAGCTTCA-3 , β-actin-F, 5 -CTCCATCATGAAGTGCGACGT-3 , β-actin-R, 5 -GTGATCTCCTTCTGCATCCTGTC-3 [1] . The quantity of vRNA was determined using a LightCycler96 (Roche, Germany) and HiScript II One Step qRT-PCR SYBR Green Kit (Vazyme, Nanjing, China), according to the instructions of the manufacturer. The relative level of RNA expression was determined by the 2 −∆∆CT method. β-actin mRNA level was used as a loading control. The mean RNA level of the mock-treated group was set at 100%.

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