Apolipoprotein D Transgenic Mice Develop Hepatic Steatosis through Activation of PPAR? and Fatty Acid Uptake

Labrie, Marilyne; Lalonde, Simon; Najyb, Ouafa; Thiery, Maxime; Daneault, Caroline; Des Rosiers, Chrisitne; Rassart, Eric; Mounier, Catherine

Supporting Information S1 Fig. ApoD expression in tissues of WT and Tg mice. The expression of H-apoD mRNA was evaluated by Northern blot analysis in various tissues of H-apoD Tg mice. HPRT was used as a reference. Results are expressed as a percentage of the expression level measured in the hippocampus and standardized by the level of HPRT expression (Fig. A) . Human apoD was quantified in plasma and liver homogenate of transgenic Tg-apoDH mice by indirect Elisa. Results are expressed as average ± SEM. (Fig. B) . Western blot analyses of plasma (P) and liver (L) in WT and Tg mice. A polyclonal mouse antibody was used to detect the endogenous apoD protein (Fig. C) .