Author: Frenzel, André; Hust, Michael; Schirrmann, Thomas
Title: Expression of Recombinant Antibodies Document date: 2013_7_29
ID: 06o7pa3d_59
Snippet: Much of the energy in transgenic production has been set on the development of humanized mice or rats. The well established property of generating hybridoma cells from these species facilitates a streamlined approach for the generation of a cell line which stably expresses monoclonal antibodies (17, 243, 244). Using humanized mice, an anti-HIV-1 gp140 antibody was identified, but in contrast the low number of antigen specific hybridomas occurring.....
Document: Much of the energy in transgenic production has been set on the development of humanized mice or rats. The well established property of generating hybridoma cells from these species facilitates a streamlined approach for the generation of a cell line which stably expresses monoclonal antibodies (17, 243, 244). Using humanized mice, an anti-HIV-1 gp140 antibody was identified, but in contrast the low number of antigen specific hybridomas occurring during the generation of the clones has been observed (245) . Therefore, the usage of humanized rats has been suggested to circumvent these problems and first antibodies have been developed with sub-nanomolar affinities using the so called OmniRat (246) .
Search related documents:
Co phrase search for related documents- antigen specific hybridoma and specific hybridoma: 1
- cell line and hybridoma cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- cell line and low number: 1, 2
- cell line and monoclonal antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37
- cell line and specific hybridoma: 1, 2, 3, 4
- cell line and transgenic production: 1, 2
- clone generation and low number: 1
- clone generation and monoclonal antibody: 1, 2
Co phrase search for related documents, hyperlinks ordered by date